Cytomegalovirus DNA, Quantitative, Real-Time PCR QCMVQT

Method(s) Real-Time Polymerase Chain Reaction (PCR)
Specimen Required
Collect

One 5 mL whole blood or plasma collected in a lavender (EDTA) top tube.

Also acceptable: yellow top (ACD) tube, plasma preparation tube (PPT), plain red, gold (SST) top tube, or 1 mL CSF or amniotic fluid collected in a sterile leak-proof container.

Transport

Transfer 1 mL whole blood or plasma (Min: 0.5 mL).

* Collection Instructions *

Whole blood:

  • Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant.
  • Store refrigerated.
  • Blood collected in tubes containing ACD anticoagulant will yield results approximately 15% lower when compared to EDTA tubes due to a dilution effect.
  • Do not freeze whole blood.

Plasma:

  • Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPTs).
  • Store collected whole blood ambient and separate plasma from cells within 2 hours of collection.
  • Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen.
  • If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen.
  • It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
  • Blood collected in tubes containing ACD anticoagulant will yield results approximately 15% lower when compared to EDTA tubes due to a dilution effect.

Serum:

  • Collect in a red top tube (no gel) or gold (SST) top tube.

CSF or amniotic fluid:

  • Collect in sterile container and store refrigerated or frozen.
Stability

Whole blood

Ambient: 48 hours

Refrigerated: 8 days

Frozen: Unacceptable

All other sample types

Ambient: 48 hours

Refrigerated: 8 days

Frozen: 30 days

Unacceptable Conditions
  • Heparinzied specimens
  • Unspun PPT tubes
Schedule Daily
Billing Code 9011142
CPTCode 87497
Notes Performing Laboratory - Quest Diagnostics Nichols Institute - Chantilly VA
Preferred Specimen Collection Device(s)
Reference Interval

CMV DNA, QN PCR - Not Detected IU/mL

CMV DNA, QN PCR - Not Detected log IU/mL

No Appointment Necessary
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12 Patient Service Centers in Central New York.

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