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Ova and Parasites, Concentrate and Permanent Smear
QOAP
Method(s) |
Microscopic Examination of Concentrate - Permanent Stained Smear |
Collect |
Stool. |
Transport |
10 grams or 10 mL fresh stool preserved in 10% formalin and polyvinyl alcohol transport or single Total-Fix transport vial. Also acceptable: - 25 mL urine in a sterile screw-cap container
- 20 mL sputum in a sterile screw-cap container
Minimum volume: 5 grams or 5 mL stool, 10 mL urine (unpreserved) 2 mL sputum or BAL |
Remarks |
* Collection Instructions * Note: Tissue samples should be sent to pathology. Stool - Place fresh stool in 10% formalin tranport vial, as well as PVA transport medium or single Total-Fix vial, within 30 minutes of collection. Add stool to bring the liquid level to the "fill to here" line on the vial. Mix well. Send specimen(s) at room temperature in the same shipping container.
- If parasite infestation is strongly suspected, collect at least 3 stool specimens every other day, since a single specimen can be negative. If Giarida is strong suspected, please see Girardia Antigen, EIA, Stool (Test Code 8625).
Urine - Urine may be submitted unpreserved for exam for Schistosoma.
- Collect at mid-day.
- Peak egg secretion occurs between noon and 3 pm.
- Do not submit first morning specimen.
- In patients with hematuria, eggs may be found trapped in blood and mucous in the terminal portion (last-voided portion) of the urine specimen.
Sputum - The specimen should be a deep expectorated sputum, preferably collected in the early morning.
- A 24-hour sputum collection is also acceptable.
- Submit in a sterile screw-cap container, unpreserved or in 10% formallin to increase stability.
Transport Container - Stool submitted 10% formalin and polyvinyl alcohol transport or single Total-Fix transport vial
- Urine submitted in a sterile screw-cap container
- Sputum submitted in a sterile screw-cap container or with 10% formalin
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Stability |
Stool (preserved) Ambient: 6 months Refrigerated: 6 months Frozen: Unacceptable Urine, Sputum, BAL (unpreserved) Ambient: Unacceptable Refrigerated: 48 hours Frozen: Unacceptable Sputum BAL (preserved) Ambient: 6 months Refrigerated: Unacceptable Frozen: Unacceptable |
Unacceptable Conditions |
- Unpreserved stool
- Specimens containing barium
- Stool preserved in medium other than parasitology fixative
- Received frozen
- Stool submitted in expired transport vial
- Preserved urine
- Unpreserved sputum and urine received ambient or frozen
- Liver abscess or aspirate
- ECOFIX transport vials
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Schedule |
Mon-Fri |
Billing Code |
9011536 |
CPTCode |
87177, 87209 |
Notes |
Performing Laboratory Quest Diagnostics-Pittsburgh Lab |
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