F-Actin (Smooth Muscle) Antibody, IgG by ELISA with Reflex to Smooth Muscle Antibody, IgG Titer FACTIN

Method(s) Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Specimen Required
Collect

One 5 mL gold (SST) top tube.

Transport

Separate from cells as soon as possible or within 2 hours of collection. Transfer1 mL serum at 2-8°C (Min: 0.25 mL)

Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Unacceptable Conditions

Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Schedule Daily
Billing Code 5010685
CPTCode 83516; if reflexed, add 86256
Notes

In viral hepatitis the titers are generally less than 1:80 and are transient. The titers in primary biliary cirrhosis are also low, ranging from 1:20 to 1:40. If F-Actin is 20 Units or greater, then Smooth Muscle Antibody, IgG IFA titer will be added.

Preferred Specimen Collection Device(s)
Reference Interval
 
ComponentsReference Interval
F-Actin (Smooth Muscle) Ab, IgG by ELISA19 Units or lessNegative
 20-30 UnitsWeak Positive - Suggest repeat testing in 2-3 weeks with fresh specimen
 31 Units or greaterPositive - Suggestive of autoimmune hepatitis or chronic active hepatitis
Smooth Muscle Antibody, IgG TiterLess than 1:20Negative - No Antibody detected
 1:20 - 1:80Weak Positive - Suggest repeat testing in 2-3 weeks with fresh specimen
 1:160 or greaterPositive - Suggestive of autoimmune hepatitis or chronic active hepatitis
No Appointment Necessary
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11 Patient Service Centers in Central New York.

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