One 3 mL light blue (sodium citrate) top tube.

1 mL frozen plasma (Min: 0.5 mL).

CRITICAL FROZEN

* Collection Instructions *

• Draw blood in a light blue-top tube containing 3.2% sodium citrate.
• Mix gently by inverting 3-4 times.
• Centrifuge 15 minutes at 1500 g within one hour of collection.
• Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
• Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s).
• Plasma must be free of platelets (<10,000/uL).
• Freeze immediately and transport on dry ice.

Ambient: Unacceptable

Refrigerated: Unacceptable

Frozen: 30 days

• Hemolysis
• Received thawed