Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA ANCAPN
| Method(s) | Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay | ||||||||||||||||||||||||||
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| Specimen Required | |||||||||||||||||||||||||||
| Collect | One 5 mL gold (SST) top tube. |
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| Transport | Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum, refrigerated (Min: 0.5 mL) |
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| Stability | (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) |
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| Unacceptable Conditions | CSF, plasma, urine or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. |
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| Schedule | Daily | ||||||||||||||||||||||||||
| Billing Code | 5011951 | ||||||||||||||||||||||||||
| CPTCode | 83516 x 2; if reflexed, add 86255 | ||||||||||||||||||||||||||
| Notes | If MPO and/or PR3 results are equivocal or positive, then ANCA by IFA will be added. Additional changes apply. Specimens are screened by IFA on ethanol-fixed neutrophils, formalin-fixed neutrophils, and HEp-2 slides that allow differentiation of C- and P-ANCA patterns. | ||||||||||||||||||||||||||
| Preferred Specimen Collection Device(s) | |||||||||||||||||||||||||||
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