Mycobacterial (AFB) Culture and Smear AFBC
Method(s) | Culture/Fluorochrome Stain |
---|---|
Specimen Required | |
Collect | Respiratory specimens. Also acceptable: Body fluid, CSF, gastric aspirate, tissue, or urine. |
Transport | Transport refrigerated. * Collection Instructions * Specimen Preparation:
Respiratory specimens:
Body Fluids or CSF:
Gastric Aspirates:
Tissue:
Urine:
|
Remarks | Specimen source required. Patient Preparation: Recommended collection: Three sputum specimens at 8-24 hour intervals (24 hours when possible) and at least one first-morning specimen. An individual order must be submitted for each specimen. |
Stability | Ambient: 24 hours; Refrigerated: 1 week, Frozen: 1 week |
Unacceptable Conditions | Dry material or material collected and transported on a wooden shaft swab. Acid Fast Stain: Stool, blood, bone marrow, grossly bloody specimens, CSF if less than 5 mL, or urine specimens if less than 40 mL. |
Schedule | Daily |
Billing Code | 3010175 |
CPTCode | 87116, 87206, 87015, 87556 |
Notes | A direct specimen PCR test for M. tuberculosis complex in sputum is automatically performed on one of three sputum samples submitted for the detection of M. tuberculosis. It is also approved for sputum specimens submitted to remove patients from respiratory isolation. Cultures are routinely held for 8 weeks. A bacterial culture, fungal culture, and AFB culture will always be performed on bronchial specimens. |
Preferred Specimen Collection Device(s) | |
Reference Interval | |
No AFB isolated. |