Tissue Transglutaminase Antibody, IgA with Reflex to Endomysial Antibody, IgA Titer EDAB

Method(s)	Multiplex Bead Immunoassay/Indirect Fluorescent Antibody
Specimen Required
Collect	One 5 mL gold (SST) top tube.
Transport	1 mL serum at 2-8°C.
Stability	After separation from cells: Ambient: 8 hours; Refrigerated: 7 days; Frozen: 1 year (avoid freeze/thaw cycles)
Unacceptable Conditions	Plasma. Contaminated, heat-inactivated or severely hemolyzed samples.
Schedule	Mon-Fri
Billing Code	2010221
CPTCode	83516 tTG IgA; if reflexed, add 86256 EMA titer
Notes	IgA class endomysial antibodies are seen in gluten-sensitive enteropathies. Approximately 70% of patients with dermatitis herpetiformis and almost 100% of patients with active celiac disease will demonstrate IgA antibodies. These antibodies generally decrease or become negative in patients on gluten-free diets and will usually reappear upon gluten challenge. All positives are titered to endpoint. If tTG IgA is 15 U/mL or greater, then EMA titer will be added.
Preferred Specimen Collection Device(s)
Gold Cap Tube with SST Gel & Clot Activator
Reference Interval
Component Reference Interval Tissue Transglutaminase Antibody, IgA Negative: less than 15 U/mL Endomysial Antibody, IgA Titer Less than 1:10