West Nile Virus Antibodies, IgG and IgM, Serum WNILES

Method(s)	Enzyme Linked Immunosorbent Assay
Specimen Required
Collect	One 5 mL gold (SST) top tube.
Transport	1 mL serum at 2-8ºC.
Stability	After separation from cells: Ambient: 2 days; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Unacceptable Conditions	Plasma. Hemolyzed, severly lipemic, heat-inactivated, or contaminated samples.
Schedule	Tue, Fri
Billing Code	5010436
CPTCode	86789, 86788
Notes	Separate serum from cells ASAP. Acute and convalescent samples must be labeled as such; parallel testing is preferred and convalescent samples MUST be received within 30 days from receipt of acute samples. Please mark sample plainly as "ACUTE" or "CONVALESCENT".
Preferred Specimen Collection Device(s)
Gold Cap Tube with SST Gel & Clot Activator
Reference Interval
Component Reference Interval West Nile Virus Antibody, IgG by ELISA, Serum Less than 1.29 IV: Negative - No significant level of West Nile virus IgG antibody detected.   1.30-1.49 IV: Equivocal - Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful.   1.50 IV or greater: Positive - Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection. West Nile Virus Antibody, IgM by ELISA, Serum Less than 0.89 IV: Negative - No significant level of West Nile virus IgM antibody detected.   0.90-1.10 IV: Equivocal - Questionable presence of West Nile virus IgM antibody detected. Repeat testing in 10-14 days may be helpful.   Greater than 1.11 IV: Positive - Presence of IgM antibody to West Nile virus detected, suggestive of current or recent infection.