Pyruvic Acid PYRUV
| Method(s) | Quantitative Enzymatic |
|---|---|
| Specimen Required | |
| Collect | Green (sodium or lithium heparin) top tube. Immediately after blood is drawn, add exactly 1 mL whole blood to a chilled pyruvate collection tube containing perchloric acid. Pyruvate collection tubes may be obtained by calling Customer Service at 315-461-3008. Collect Monday - Friday Only. Mix well for 30 seconds then place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1500 x g. Decant 2 mL supernatant into a standard transport tube and freeze. |
| Transport | 2 mL supernatant, frozen. (Min: 1 mL) CRITICAL FROZEN. |
| Remarks | Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet. |
| Stability | Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks |
| Unacceptable Conditions | If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing to be performed. |
| Schedule | Daily |
| Billing Code | 5010825 |
| CPTCode | 84210 |
| Preferred Specimen Collection Device(s) | |
| Reference Interval | |
|
0.030-0.107 mmol/L (venous blood) |
|
