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Testosterone by LC/MS/MS and Associated Batteries: Free and Total Testosterone, LC/MS/MS and Bioavailable Testosterone, LC/MS/MS - New Test Bulletin

Thursday, January 3, 2019

Effective January 8, 2019, Laboratory Alliance of Central New York will send out specimens for Testosterone testing by Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS) to our reference lab (ARUP Laboratories).  In addition, testosterone evaluation batteries for assessment of free and bioavailable testosterones that involve the measurement of total testosterone by LC-MS/MS will also be sent out to our reference lab.

Good agreement can be expected between ARUP’s LC-MS/MS method and our in-house LC-MS/MS method (which will be discontinued) because both are based on LC-MS/MS technology, which is considered the gold standard for measuring testosterone in women and children.

The circulating concentration of testosterone in women is only 5-10% of that in men.  In children, the circulating concentration of testosterone is highly age and sex dependent, with reference ranges extending down to less than 5% of the adult male testosterone concentration.   Testosteronetesting by LC-MS/MS methodology is necessary for the accurate quantitation of the lower concentrations of testosterone found in samples from women and children.  Testosterone testing by immunoassay methodology is suitable for accurate quantitation of the higher concentrations of testosterone in samples from adult males.  Laboratory Alliance will continue to perform testosterone testing in-house on our automated immunoassay platform.

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