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Anatomic Pathology

Cytopathology Services for St. Joseph's Hospital Health Center

CYTOPATHOLOGY
TYPE OF SPECIMEN	RECOMMENDATIONS	LIMITATIONS/REJECTIONS
CONVENTIONAL PAP SMEAR	1 slide pap smear, labeled in pencil, collected with cervix brush, cytobrush or spatula, mono-layer smear fixed with 95% ETOH or spray fix.	Unlabeled, mislabeled, air dried or formalin fixation.
THIN PREP PAP SMEAR	Labeled thin prep vial, collected by cervix brush or cytobrush/spatula - rinse collection device well in fixative.
BREAST DISCHARGE SMEAR	Collect discharge directly on a pencil-labeled slide. Fix immediately with 95% ETOH or spray fix.	Air dried specimen, formalin fixation.
BRONCHIAL WASH	Fresh washing, submitted in leak-proof labeled specimen container. Should be refrigerated if collected outside of lab operation hours.
BRUSHINGS (BRONCHIAL, GASTRIC, ESOPHAGEAL, ETC.)	Roll brush on slide labeled in pencil. Fix immediately in 95% ETOH.	Air dried specimen, formalin fixation.
CSF	At least 1 ml. in labeled leak-proof container. Refrigerate if collected outside of lab hours.
FINE NEEDLE ASPIRATIONS	Assistance from Cytotech's making slides Rapid Adequacy Evaluation in Radiology Specialty Services or on patient floors. Outreach Aspirations may be collected in fixative available from Cytopathology lab.
SEROUS FLUIDS: ASCITES, PLEURAL, PERICARDIAL	Submit in labeled, leak-proof container (90 ml. Specimen cup). Refrigerate in off hours. More than 5 ml. Preferred.
SPUTUMS	Deep cough specimen collected before breakfast. Induced sputa may be collected any time if the patient has not eaten. Specimen container must be labeled.	If pulmonary macrophages are not identified - the specimen will be reported as unsatisfactory
TZANCK SMEARS FOR HERPES	Scraping from base of lesion, spread in monolayer on a labeled slide. FIX IMMEDIATELY in 95% ETOH.	Air dried smear or formalin fixation.
URINES	A second morning, voided specimen is suggested. Specimen should be submitted in labeled, leak-proof container. Specimen should be refrigerated in off hours.
CONSULTATION ON OUTSIDE CASES	Glass slides and a copy of the referring laboratory report.	The attending physician may directly request the necessary material from an outside laboratory or may ask the Cytology Department to do so. (315-410-7210)